protease activated receptor 4 par4 Search Results


93
Alomone Labs par 4 antibody
Par 4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protease+activated+receptor+4+par4/pm33529332-26-14-18?v=Alomone+Labs
Average 93 stars, based on 1 article reviews
par 4 antibody - by Bioz Stars, 2026-07
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93
Alomone Labs anti par4 fitc antibody
Flow cytometric analysis of <t>PAR4</t> expression in wild type (WT) (black line), PAR3 −/− (gray line), and PAR4 −/− (shaded) mice platelets using <t>anti-PAR4-FITC</t> antibodies.
Anti Par4 Fitc Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protease+activated+receptor+4+par4/pmc03566007-45-1-6?v=Alomone+Labs
Average 93 stars, based on 1 article reviews
anti par4 fitc antibody - by Bioz Stars, 2026-07
93/100 stars
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90
Alomone Labs par 4
Flow cytometric analysis of <t>PAR4</t> expression in wild type (WT) (black line), PAR3 −/− (gray line), and PAR4 −/− (shaded) mice platelets using <t>anti-PAR4-FITC</t> antibodies.
Par 4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protease+activated+receptor+4+par4/10__1189_slash_jlb__3ab1013___567r-79-32-36?v=Alomone+Labs
Average 90 stars, based on 1 article reviews
par 4 - by Bioz Stars, 2026-07
90/100 stars
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N/A
Protease Activated Receptor 4 Antibody is a Rabbit Polyclonal against Protease Activated Receptor 4
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Image Search Results


Flow cytometric analysis of PAR4 expression in wild type (WT) (black line), PAR3 −/− (gray line), and PAR4 −/− (shaded) mice platelets using anti-PAR4-FITC antibodies.

Journal: PLoS ONE

Article Title: Calcium Mobilization And Protein Kinase C Activation Downstream Of Protease Activated Receptor 4 (PAR4) Is Negatively Regulated By PAR3 In Mouse Platelets

doi: 10.1371/journal.pone.0055740

Figure Lengend Snippet: Flow cytometric analysis of PAR4 expression in wild type (WT) (black line), PAR3 −/− (gray line), and PAR4 −/− (shaded) mice platelets using anti-PAR4-FITC antibodies.

Article Snippet: The anti-PAR4-FITC antibody was purchased from Alamone Labs Ltd. (Jerusalem, Israel).

Techniques: Expressing

The HEK293 cells were transfected with: ( A ) PAR4-Luc (1 µg) and PAR3-GFP (0–2.5 µg), ( B ) PAR3-Luc (1 µg) and PAR3-GFP (0–2.5 µg), or ( C ) PAR4-Luc (1 µg) and PAR4-GFP (0–2.5 µg). As a control experiment, the HEK293 cells were transfected with: ( D ) PAR3-Luc (1 µg) and rho-GFP (0–0.12 µg), or ( E ) PAR4-Luc (1 µg) and rho-GFP (0–0.12 µg). Forty-eight hours post-transfection, the cells were analyzed for GFP expression, Luc expression, and BRET. The curves were plotted as the ratio of GFP to Luc and all points from 3–6 independent experiments were analyzed by global fit to a hyperbolic or linear curve. The surface expression of PAR3 and PAR4 in the HEK293 cells was determined by flow cytometry. ( G ) V5-PAR4-GFP and V5-PAR3-GFP were detected with a V5 tag antibody conjugated to Alexa Fluor 647. ( H ) HA-PAR4-LUC and HA-PAR3-LUC were detected with a HA tag antibody conjugated to Alexa Fluor 647. The results are the mean (± SD) of two independent experiments. The number of PAR4 and PAR3 molecules on the HEK293 cells surface is calculated from the V5 or HA antibody standard curve using quantitative flow cytometry ( F ).

Journal: PLoS ONE

Article Title: Calcium Mobilization And Protein Kinase C Activation Downstream Of Protease Activated Receptor 4 (PAR4) Is Negatively Regulated By PAR3 In Mouse Platelets

doi: 10.1371/journal.pone.0055740

Figure Lengend Snippet: The HEK293 cells were transfected with: ( A ) PAR4-Luc (1 µg) and PAR3-GFP (0–2.5 µg), ( B ) PAR3-Luc (1 µg) and PAR3-GFP (0–2.5 µg), or ( C ) PAR4-Luc (1 µg) and PAR4-GFP (0–2.5 µg). As a control experiment, the HEK293 cells were transfected with: ( D ) PAR3-Luc (1 µg) and rho-GFP (0–0.12 µg), or ( E ) PAR4-Luc (1 µg) and rho-GFP (0–0.12 µg). Forty-eight hours post-transfection, the cells were analyzed for GFP expression, Luc expression, and BRET. The curves were plotted as the ratio of GFP to Luc and all points from 3–6 independent experiments were analyzed by global fit to a hyperbolic or linear curve. The surface expression of PAR3 and PAR4 in the HEK293 cells was determined by flow cytometry. ( G ) V5-PAR4-GFP and V5-PAR3-GFP were detected with a V5 tag antibody conjugated to Alexa Fluor 647. ( H ) HA-PAR4-LUC and HA-PAR3-LUC were detected with a HA tag antibody conjugated to Alexa Fluor 647. The results are the mean (± SD) of two independent experiments. The number of PAR4 and PAR3 molecules on the HEK293 cells surface is calculated from the V5 or HA antibody standard curve using quantitative flow cytometry ( F ).

Article Snippet: The anti-PAR4-FITC antibody was purchased from Alamone Labs Ltd. (Jerusalem, Israel).

Techniques: Transfection, Control, Expressing, Flow Cytometry